Autoclave Temperature – Check the temperature in the bag

One of the foremost important parts today is that the autoclave temperature. Autoclave bags are accustomed sterilize infectious tools and waste. The autoclave steam sterilization process relies on monitoring three parameters: time, temperature, and pressure. It uses heat to perform sterilization and carries moisture within the correct amount. to attain an efficient sterilization process, the control of every of the three parameters must be combined to supply saturated steam. A controlled process will produce the precise percentage of saturation that may carry the warmth onto the microbes. The cycle requirements for each load type can, however, vary significantly. The three sterilization cycles are Gravity, Pre-vacuum, and Air Steam Mixture Cycle.

Autoclave Temperature, pressure and time differences in decontamination

The Gravity Cycle is the simplest; ideal for sterilizing liquids, media, glassware & plastic, culture plates, and unwrapped instruments.

The cycle begins with a brief heating phase as steam is introduced into the chamber. As steam fills the chamber, the air is forced out through a drain vent. By pushing the air out, the steam directly contacts the load and begins to sterilize it. Sterilization occurs when a pressure of 208 kPa and a temperature of 121℃ is reached. The sterilization phase is approximately 1000 seconds long. Afterward, the system and cargo are cooled down at a awfully slow pace that may take up to 10 hours. The slow cool-down prevents liquids from boiling over and spilling. there’s an choice to add a fast cooling system that decreases cycle time by up to 80%

Property of Time Temperature Perisher Autoclave bags
gravity cycle
Autoclave Temperature

Used to Sterilize 

• Liquids
• Media
• Glassware and plastic – without hollows
• Culture Plates
• Unwrapped Instruments


• Incomplete Steam Penetration
• Wet Loads at End of Cycle (not ideal for soft packaging)
• Longer duration with high load volume, not recommended for hollow or porous loads
• Sealed liquid parenteral in polymer packaging


 • Ideal for sterilizing liquids
• Very simple process
• Low cost autoclave
pre vacuum cycle graph
Autoclave Temperature

The Pre-vacuum cycle actively removes air from the chamber before the sterilization phase, employing a air pump. it’s used for sterilizing hollow loads, porous loads, and wrapped packages. The cycle begins with 4 pulses of vacuum, gradually removing air from the chamber and inserting steam into it. Eventually, over 99% of the air is removed and that we are left with 0.016% of initial air (after 400 seconds within the above graph). at that time we gradually heat the chamber, creating an identical temperature throughout the degree. Sterilization then occurs (Seconds 1000 to 1200).

After sterilization, the chamber and cargo are cooled down. When the pressure drops, all residual moisture boils and is transformed into a gaseous state. When pressure is released from the chamber, the moisture exits further, leaving our product sterilized and dry.

Used to Sterilize

• Porous loads
• Hollow loads
• Solid loads
• Wrapped packages


• Air removal of more than 99%
• Uniform heat distribution and temperature homogeneity


• Cannot sterilize liquid
pre vacuum cycle graph
Autoclave Temperature

The Air Steam Mixture Cycle (ASM) is employed to sterilize liquids that are sealed in a very package. These include contact lenses, glass bottles, ampoules, and infusion bags. it’s designed to sterilize large quantities; for instance, 25,000 ampoules, 20,000 infusion bags, or 30,000 syringes. Packaging integrity is maintained and there’s no deformation of elastic packages.

In the ASM cycle, the sterilization media may be a mixture of steam and air; i.e., air removal isn’t a problem because the loads are sealed (Ampule for example). Throughout the cycle overpressure is maintained; the pressure external to the load is more than internal pressure. Temperature is gradually increased to realize a uniform temperature increment (Seconds 0-2000 within the above graph). This state of overpressure is maintained throughout the sterilization process. After the sterilization cycle ends, the temperature is slowly reduced by water that’s introduced into the jacket. We start the cooling using air while maintaining constant high and thus avoiding boiling (low pressure means lower boiling temperature). Once the temperature is below 80℃, the air is released and therefore the load is prepared for usage. The result’s a sterile product that’s dry and prepared for packaging. those that are autoclave bags wholesale can contact us.


• Aqueous solutions in sealed containers
• Glass bottles
• Bags
• Vials / Ampoules
• LVS – Large volume syringes
• PFS – Pre-filled syringes
• Contact Lenses


• Uniform heat penetration (heating/hold stage)
• Packaging integrity is maintained
• No deformation of elastic packages
• Avoidance of direct steam exposure
focus on autoclave bags
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